Catalog Number - 59034-15P-3

Axis Cyano™ is a versatile cyanopropyl HPLC stationary phase that provides unique selectivity for compounds that may not be adequately resolved on conventional C18 columns. Manufactured on high-purity silica with a bonded 3-carbon ligand terminating in a cyano functional group, this stationary phase has been a trusted choice in analytical laboratories for many years. Its moderate polarity makes it useful for a broad range of reversed phase applications while providing retention characteristics distinct from traditional alkyl-bonded phases.

59034-15P-3 is a 150 mm x 4.6 mm ID analytical column packed with 3 µm, 100Å Axis Cyano particles. The 150 mm length provides an excellent balance of resolution and run time, while the smaller 3 µm particle size delivers greater efficiency, sharper peaks, and improved chromatographic performance compared to 5 µm particles. The industry-standard 4.6 mm ID format typically operates at approximately 1.0–2.0 mL/min on conventional HPLC systems; however, users should expect higher backpressure due to the smaller particle size.

This column is an excellent alternative for methods specifying USP L10 columns. The cyano stationary phase is commonly used for polar and neutral compounds in reversed phase chromatography and typically exhibits shorter retention times and faster equilibration than unmodified silica columns. Packed and shipped in reversed phase solvents, this column is optimized for reversed phase operation. Laboratories requiring normal phase cyano applications should select columns specifically packed in normal phase solvents.

Key Benefits

• USP L10 cyano stationary phase

• Higher efficiency than comparable 5 µm columns

• Sharper peaks and improved resolution

• Unique selectivity compared to traditional C18 columns

• Suitable for polar and neutral compounds

• Faster equilibration than unmodified silica

• High-purity silica support

• Compatible with conventional HPLC systems

Typical Applications

• USP L10 methods

• Reversed phase HPLC

• Pharmaceutical analysis

• Impurity profiling

• Method development

• Polar compound separations

• Neutral compound analysis

• Alternative selectivity screening

Specifications

• Product Type: HPLC Column

• Stationary Phase: Axis Cyano

• USP Classification: L10

• Particle Size: 3 µm

• Pore Size: 100Å

• Bonded Phase: Cyanopropyl

• Endcapped: No

• Column Dimensions: 4.6 mm ID x 150 mm

• Length: 150 mm

• Recommended Flow Rate: 1.0–2.0 mL/min

• Brand: Cogent

Resources

• View Axis Cyano Columns Packed in Normal Phase Solvents

Important Notes

• Packed and shipped in reversed phase solvents

• Designed primarily for reversed phase chromatography

• USP L10 column classification

• Smaller particle size provides greater efficiency and sharper peaks

• Industry-standard 4.6 mm ID analytical format

• 150 mm length balances resolution and analysis time

• Higher operating pressures than comparable 5 µm columns

  ---